Our work group´s “Romanian branch”, led by Lucian Copolovici had published a methods paper, suggesting two novel methods how to capture and measure volatile organic compounds in plants, together with Astrid Kännaste and Ülo Niinemets from our lab.
Full citation: Copolovici, L., Kännaste, A., Pag, A., Tomescu, D., Bodescu, A., & Niinemets, Ü. (2015). Volatile Organic Compounds Emitted by Plants Determination using New Gas Chromatography Mass-Spectrometry Methods. Scientific Bulletin of ESCORENA, 10, 31-36. (link to full text)
In the nature, plants emit numerous volatile organic compounds. Common plant volatiles include various green leaves volatiles, terpenes, phenylpropanoids and/or benzenoids. In the present paper it will be characterized thermal desorption (TD) and solid phase microextraction methods (SPME) for simultaneous determination of green leaves volatiles (GLVs), various mono- and sesquiterpenes in headspace of plants. The first method is based on preconcentration of VOCs on solid absorbents coupled with the gas chromatograpy mass-spectrometry coupled with thermal desorption system (GCMS-TD). For trapping the volatile organic compounds (VOC) we used a multibead tube filled with solid sorbents (Carbotrap c and Carbopack c). Different types of solid sorbents have been tested and characterised. The second method is based on adsorption of different volatile compounds on the fibres followed by GC-MS analyses. The fibres trapped and released volatile organic compounds with different numbers of carbons atoms. Both methods have been used for volatile organic compounds emitted by plants from Betulaceae family.